Part:BBa_K2622039:Experience
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2622039
Amplified gene sequence of BamA was ligated into pET28b plasmid. Protein expression was induced by 0,1mM IPTG and overexpressed in cytoplams in a form of inclusion bodies. Bacteria cells were lysed by sonication, inclusion bodies were isolated by centrifugation and dissolved in 8M urea. This yielded high purity of BamA and therefore did not need any further purification. BamA was used in a folding assay, which have shown that BamB and BamCDE lipoproteins can assemble BamA into the membrane with an efficiency higher than 50% in time range of only two hours.
User Reviews
UNIQdd1c5eea96db8a46-partinfo-00000000-QINU UNIQdd1c5eea96db8a46-partinfo-00000001-QINU